University of Florida

John J. Bromfield, Ph.D.

Our laboratory focuses on infection and immunity in the female reproductive tract, in particular we have a specific interest in uterine infection in dairy cows. Approximately 40% of all dairy cows acquire a uterine infection following calving resulting in pain, infertility and reduced milk production resulting in an annual cost to the US dairy industry of $650 million.
Using molecular and cellular techniques we aim to elucidate the mechanisms involved in the innate immune response to invading pathogens in the uterus resulting in infertility. The Gram-negative bacteria E. coli, comprising a lipopolysaccharide (LPS) cell wall, is the primary causes of clinical metritis in dairy cows. The innate immune response to LPS is orchestrated through the Toll-like receptor (TLR) pathway. Interestingly, dairy cows with uterine infections accumulate LPS in the ovarian follicle and initiates an inflammatory response by the ovarian granulosa cells. This response results in reduced oocyte quality while reducing the follicular reserve and can explain some level of infertility in cows with infections.
We aim to understand how LPS, and other bacterial components negatively impacts the ovary and oocyte. By understanding these mechanisms we will be able to intervene in dairy cows with infection and promote fertility and milk production.
Secondary to infection and immunity our lab has an active interest in improving oocyte cryopreservation and the developmental environment of the early embryo.

Representative Publications:
JC Price, JJ Bromfield, IM Sheldon. Pathogen-associated molecular patterns initiate inflammation and perturb the endocrine function of bovine granulosa cells from ovarian dominant follicles via TLR2 and TLR4 pathways.Endocrinology 154 (9), 3377-3386, 2013.

JJ Bromfield, IM Sheldon. Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo. Biology of Reproduction 88 (4), 2013.

JJ Bromfield, IM Sheldon. Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4 pathway and perturbs oocyte meiotic progression in vitro. Endocrinology 152 (12), 5029-5040, 2011.

IM Sheldon, JJ Bromfield. Innate immunity in the human endometrium and ovary. American Journal of reproductive Immunology 66 (s1), 63-71, 2011.

M McLaughlin, JJ Bromfield, DF Albertini, EE Telfer. Activin promotes follicular integrity and oogenesis in cultured pre-antral bovine follicles. Molecular Human Reproduction 16 (9), 644-653, 2010

IM Sheldon, AN Rycroft, B Dogan, M Craven, JJ Bromfield, et al. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. PloS One (2), e9192, 2010.

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broomfield

John J. Bromfield, Ph.D.
Assistant Professor
Department of Animal Sciences
Ph.D., University of Adelaide
Postdoc: Swansea University; Univ. Missouri