University of Florida

Dr. John Bromfield

Assistant Professor

Fall 2016 Course

Research Summary

Our laboratory focuses on infection and immunity in the female reproductive tract, in particular we have a specific interest in uterine infection in dairy cows. Approximately 40% of all dairy cows acquire a uterine infection following calving resulting in pain, infertility and reduced milk production resulting in an annual cost to the US dairy industry of $650 million.

Using molecular and cellular techniques we aim to elucidate the mechanisms involved in the innate immune response to invading pathogens in the uterus resulting in infertility. The Gram-negative bacteria E. coli, comprising a lipopolysaccharide (LPS) cell wall, is the primary causes of clinical metritis in dairy cows. The innate immune response to LPS is orchestrated through the Toll-like receptor (TLR) pathway. Interestingly, dairy cows with uterine infections accumulate LPS in the ovarian follicle and initiates an inflammatory response by the ovarian granulosa cells. This response results in reduced oocyte quality while reducing the follicular reserve and can explain some level of infertility in cows with infections.

We aim to understand how LPS, and other bacterial components negatively impacts the ovary and oocyte. By understanding these mechanisms we will be able to intervene in dairy cows with infection and promote fertility and milk production.

Secondary to infection and immunity our lab has an active interest in improving oocyte cryopreservation and the developmental environment of the early embryo.


2002-2006; PhD (Medicine), University of Adelaide, Australia.

2001; BSc (Hons) – Reproductive Immunology, University of Adelaide, Australia.

1998-2000; Bachelor of Science (Double major Biochemistry and Immunology), University of Adelaide, Australia.

Academic Positions Since Final Degree:

2014-current; Assistant Professor of Physiology. Department of Animal Sciences, University of Florida.

2012-2014; Postdoctoral fellow. Department of Obstetrics, Gynaecology and Women’s Health, University of Missouri-Columbia.

2009-2012; Research Officer. Institute of Life Science, School of Medicine, Swansea, UK.

2008-2009; Lead Teaching Assistant. Frontiers in Reproduction course. Marine Biological Laboratories, Woods Hole, MA.

2007-2009; Postdoctoral fellow. Department of Molecular and Integrative Physiology, Kansas University Medical Centre.

2006-2007; NHMRC Postdoctoral Research Officer. Department of Obstetrics and Gynaecology, University of Adelaide, Australia.

Professional Memberships

Society for the Study of Reproduction (SSR)
European Society of Human Reproduction and Embryology (ESHRE)
Society for Reproduction and Fertility (SRF)
International Society for Fertility Preservation (ISFP)
Frontiers in Reproduction Firbee

Honors and Recognition

2013; University of Adelaide international exchange fellow.

2010; Wales Obstetrics and Gynaecology Society communication prize.

2009; International Society for Fertility Preservation communication prize.

2005; Foetal and Neonatal Physiological Society, DSL Young Investigator Award.

2005; Australian Foetal and Neonatal Physiology Workshop communication prize.



John Bromfield, Ph.D.

PO Box 110910
Gainesville, FL 32611
Phone: 352-392-1981 Ext. 241
Fax: 352-392-5595

2250 Shealy Dr.
Gainesville, FL 32611