ANS 6905 Experimental Embryology
Credits 4.0
Lecture MWF
10:40-11:30 am
Laboratory R 12:50-2:45 pm
INSTRUCTOR: Dr. Karen Moore, Assistant Professor
Office:
204F Bldg. 499 Shealy Drive
Phone:
392-1958
Fax: 392-5595
Web: http://www.animal.ufl.edu/moore/ans-6905.html
Office
Hours: By Appointment
EFFECTIVE TERM: Fall 2002
COURSE DESCRIPTION: Fundamentals of embryology with emphasis on
current experimental approaches utilized in research of normal and abnormal
development of the mammalian embryo.
PREREQUISITES: ANS 6751,
BCH 5045.
COURSE FORMAT: Three one-hour lectures and one 2-hour lab per
week. The course will be taught as a
series of lectures and discussions in order to develop a general understanding
of embryology and the experimental systems for studying early embryonic
development. The lab will be devoted to
hands-on demonstrations to emphasize topics covered in the lecture each week.
Students will be required to critically review recent literature,
prepare a research proposal utilizing some aspect of embryology described in
the course, and give an oral presentation related to their proposal.
COURSE OBJECTIVES:
1) To develop an understanding of the processes of embryonic
development.
2) To review current methodologies for conducting research in the
field of embryology.
3) To get hands-on experience during lab demonstrations.
4) To discuss and critically review the current literature in the
field of embryology.
5) To explore interests in the field of embryology and design an
experiment supported by current literature.
RECOMMENDED TEXT: There is no required text for this course. Selected reading assignments will be taken
from the following textbooks:
Developmental Biology. Scott F. Gilbert. Fifth Edition.
The Developing Human:
Clinically Oriented Embryology. Keith L. Moore.
Fourth Edition.
Handbook of In Vitro
Fertilization. Alan O. Trounson and David K. Gardner. Second Edition.
Clinical Embryology: A Color
Atlas and Text. Murray Brookes and
Anthony Zietman.
READING: Selected papers
from the current literature will also be assigned and discussed during the
semester to illustrate each of the topics of the course.
GRADES: There will be
three comprehensive take-home exams given over the semester which will apply
the knowledge gained over the semester from lectures, labs, discussions, and
assigned reading, each worth 20% of the total grade. Additionally, there will
be a term paper worth 15%, proposal presentation worth 10% of the grade and
class participation worth 5% of grade.
Grading Scale: A (90-100), B+ (87-89), B (80-86), C+ (77-79), C
(70-76), D+ (67-69), D (60-66), E (less than 60).
ANS 6905 Lecture/Lab Schedule
Aug
26 Introduction
Aug
28 Molecular
Biology Review
Aug
30 Molecular
Biology Review
Lab Sterile
Technique and Intro to Moving Eggs
Sep
2 No Class;
Labor Day
Sep
4 Cell Cycle
Sep
6 Follicular
Development, Ovulation and Fertilization
Lab In Vitro
Maturation
Sep
9 In Vitro
Fertilization
Sep
11 In Vitro
Culture
Sep
13 IVP Paper
Discussion
Lab In Vitro
Fertilization and Embryo Culture
Sep
16 Preimplantation
Development
Sep
18 Preimplantation
Development
Sep
20 Intracytoplasmic
Sperm injection/ART
Lab Freezing Embryos
Sep 23 Maternal Zygotic
Transition
Sep 25 Imprinting
Sep 27 X inactivation
Lab Gene Detection by PCR
Sep 30 Exam Review/Discuss
Project Proposal Take home Midterm
Oct 2 Cell lineages
Oct 4 Sex Determination
and Gametogenesis Midterm #1 Due
Lab PGC Isolation and Fetal Sexing
Oct 7 Germ Cells and PGCs
Oct 9 Transgenesis
Oct 11 Discuss Midterm #1 Proposal
Title Due
Lab Pronuclear Microinjection
Oct 14 Transgenesis
Oct 16 Transgenesis
Oct 18 Embryonic Stem Cells
Lab ES cells
Oct 21 Embryonic Stem Cells
and Gene Targeting
Oct 23 Gene Targeting
Oct 25 Gastrulation
Lab Gene Targeting and Electroporation
Oct 28 Exam Review Take
home Midterm
Oct 30 Neurulation and Neural Crest
Nov 1 Paper Discussion Midterm #2 Due
Lab Mouse Superovulation and Embryo
Collection
Nov 4 Mesoderm and
Endoderm
Nov 6 Morphogenesis and Pattern
Formation
Nov 8 Discuss Midterm #2;
Set Debate teams
Lab Ultrasound/OPU; Pick Debate topics
Nov 11 No Class; Veteran’s
Day
Nov 13 Nuclear Transfer and
Large Offspring Syndrome Abstracts
Due
Nov 15 No Class; Homecoming
Lab Nuclear Transfer Demo
Nov 18 Placentation
Nov 20 Teratology and
Toxicology Assays
Nov 22 Paper Discussion
Lab Animal Welfare/Ethics Debates
Nov 25 Presentations
Nov 27 Presentations
Nov 29 No Class; Thanksgiving
Lab No Class; Thanksgiving
Dec 2 Exam Review Take
home Midterm
Dec 4 Presentations
Dec 6 Presentations Midterm #3 Due
Lab Presentations
Dec 9 Discuss Midterm #3
Dec
11 Last day of
Class; Projects Due