University of Florida

In Vitro Production Protocol

In Vitro Production of Bovine Embryos

The procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC) of embryos described here are based on procedures developed in other laboratories at the University of Wisconsin (Neal First), and University of Missouri (R. Michael Roberts) and University of Guelph (W. Allan King).

Keep in mind that the protocols described here are not fixed but rather constantly evolve as new developments take place.  Therefore, practitioners of IVP will be well advised to experiment with the procedures used, especially after reading of improvements made by other laboratories.

The protocol is organized as a series of pdf files that contain information for setting up the laboratory, procedures for conducting IVP, and other useful protocols for IVP.  The combined manual was produced by merging all the individual files. Access the IVP Manual Here