In Vitro Production Protocol

In Vitro Production of Bovine Embryos

The procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC) of embryos described here are based on procedures developed in other laboratories at the University of Wisconsin (Neal First), and University of Missouri (R. Michael Roberts) and University of Guelph (W. Allan King).

Keep in mind that the protocols described here are not fixed but rather constantly evolve as new developments take place.  Therefore, practitioners of IVP will be well advised to experiment with the procedures used, especially after reading of improvements made by other laboratories.

The protocol is organized as a series of pdf files that contain information for setting up the laboratory, procedures for conducting IVP, and other useful protocols for IVP.  The combined manual was produced by merging all the individual files. Access the IVP Manual Here 

• Other Useful Information

  • Helpful hints (.pdf 58KB)
  • Guide on use of instruments for picking up oocytes and embryos (.pdf 77KB)
  • Abbreviated protocol for the experienced user (.pdf 63KB)
  • Alternative protocol for processing oocytes (.pdf 66KB)
  • Alternative sperm purification procedures (.pdf 139KB)
  • Fertilization in drops (.pdf 63KB)
  • In vitro fertilization with sex-sorted semen (.pdf 55KB)
  In addition, links to other information that will be helpful for doing IVP (images, videos, other embryo protocols, etc.) are located on the right column under the heading UF IVP Links.

  Return to top